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anti human pak1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti human pak1
    Anti Human Pak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+clathrin/Clathrin+Heavy+Chain+XP+Rabbit+mAb/pm37806990-227-67-68
    Average 95 stars, based on 169 article reviews
    anti human pak1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Labeling:

    Article Title: CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion
    Article Snippet: The following mouse anti-human monoclonal antibodies were used for flow cytometry, imaging cytometry and confocal analysis of MoDCs: HLA-DR (clone L243), CD11c (B-ly6), CD103/αE (B-Ly7,) CD3 (HIT3a), CD19 (SJ25C1), CD45 (2D1), CD56 (MY31), E-cadherin (67A4), CD49d (9F10) purchased from eBioscience, Biolegend, or Tonbo, all San Diego, CA. .. Endosomal compartments were labeled with rabbit anti-human clathrin (D3C6, Cell Signaling, Danvers, MA), rabbit anti-human EEA-1 (polyclonal), mouse anti-human Rab7a (Rab7-117), and rabbit anti-human Rab11 (polyclonal), all from Abcam, Cambridge, MA. .. The following monoclonal antibodies were used for staining of paraffin-embedded tissue sections: anti-human HLA-DR (LN-3) and anti-human CD103 [EPR4166(2)], both Abcam, Cambridge, MA.



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    Partial co-localization of CD103 with endosomal markers in human MoDCs. MoDCs generated in medium alone or in the presence of retinoic acid (RA, 100 nM) were permeabilized and stained with antibodies to CD103 (green) and the following endosomal markers (red): (a–c) <t>clathrin,</t> (e–g) EEA-1, (i–k) Rab11, and (m–o) Rab7a. Images were obtained by confocal microscopy. Co-localization of CD103 with (d) clathrin, (h) EEA-1, (l) Rab11, and (p) Rab7a was calculated as the Manders' colocalization coefficient (M2) using ImageJ. Mean ± SEM of 10 −17 confocal images obtained from two independent experiments. Bar = 20 μm, * P ≤ 0.05; ** P ≤ 0.01; unpaired Student's T -test.
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    Image Search Results


    Partial co-localization of CD103 with endosomal markers in human MoDCs. MoDCs generated in medium alone or in the presence of retinoic acid (RA, 100 nM) were permeabilized and stained with antibodies to CD103 (green) and the following endosomal markers (red): (a–c) clathrin, (e–g) EEA-1, (i–k) Rab11, and (m–o) Rab7a. Images were obtained by confocal microscopy. Co-localization of CD103 with (d) clathrin, (h) EEA-1, (l) Rab11, and (p) Rab7a was calculated as the Manders' colocalization coefficient (M2) using ImageJ. Mean ± SEM of 10 −17 confocal images obtained from two independent experiments. Bar = 20 μm, * P ≤ 0.05; ** P ≤ 0.01; unpaired Student's T -test.

    Journal: Frontiers in Immunology

    Article Title: CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion

    doi: 10.3389/fimmu.2018.02989

    Figure Lengend Snippet: Partial co-localization of CD103 with endosomal markers in human MoDCs. MoDCs generated in medium alone or in the presence of retinoic acid (RA, 100 nM) were permeabilized and stained with antibodies to CD103 (green) and the following endosomal markers (red): (a–c) clathrin, (e–g) EEA-1, (i–k) Rab11, and (m–o) Rab7a. Images were obtained by confocal microscopy. Co-localization of CD103 with (d) clathrin, (h) EEA-1, (l) Rab11, and (p) Rab7a was calculated as the Manders' colocalization coefficient (M2) using ImageJ. Mean ± SEM of 10 −17 confocal images obtained from two independent experiments. Bar = 20 μm, * P ≤ 0.05; ** P ≤ 0.01; unpaired Student's T -test.

    Article Snippet: Endosomal compartments were labeled with rabbit anti-human clathrin (D3C6, Cell Signaling, Danvers, MA), rabbit anti-human EEA-1 (polyclonal), mouse anti-human Rab7a (Rab7-117), and rabbit anti-human Rab11 (polyclonal), all from Abcam, Cambridge, MA.

    Techniques: Generated, Staining, Confocal Microscopy